2026-10-11T14:30:50Z https://njump.cloud.vinney.xyz MCDuncanLab https://njump.cloud.vinney.xyz/npub14k3sv7gtyhujmep00nkx86m4h6yv2rsna740rasupl93h00d2fvq26yep4 https://media.mstdn.social/accounts/avatars/109/269/922/679/986/135/original/b26c540e66afc9b7.jpg https://media.mstdn.social/accounts/avatars/109/269/922/679/986/135/original/b26c540e66afc9b7.jpg https://njump.cloud.vinney.xyz/nevent1qqsyxkkudcqnhg2aahgc5tzzssv5mnn4cdhhzayj9c8l9mvzj5qunwczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9ssjyt6x Mid October and we’re still getting peppers, beans, and eggplants.<br/><br/>The beans are probably done for with a frost later this week but the eggplant and peppers can be covered. Gotta pick who gets to over winter inside soon.<br/><br/>Fall mushrooms are going strong.<br/><br/>#gardening<br/> <img src="https://media.mstdn.social/media_attachments/files/117/436/396/542/374/970/original/78d73894a2fc5e72.png"> <br/> 2026-10-14T00:22:06Z https://njump.cloud.vinney.xyz/nevent1qqsyxn82s5t5lflkgh9xsnydetgyt203hlagky4wkj9h6zrcv2ndafczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9s83pxuu In reply to <a href='/nevent1qqsgzycdl7pfyh55xlww4x35cgv889frk6tgv3ktz2gg0y4n8mrxnrq7m27v0'>nevent1q…27v0</a><br/>_________________________<br/><br/>Gosh that hits home so hard. Having just traveled with my 77 yo mom who is still healthy and thinking how little time we have left. She had me when she was 23 my brother one year later. <br/><br/>A 46 yo parent, will have kids just barely in their 30s when they hit 77. I wasn’t in a place where I could pay to ditch off for two weeks with mom in my early 30s.<br/><br/>Maybe if you grow up with older parents you come to that realization earlier than me, I do hope so. 2026-10-11T15:10:35Z https://njump.cloud.vinney.xyz/nevent1qqsp5t09edh6rlfyq6ea7k0t7s8zujrmm285hr09qgej6f9hl80yp6czyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9smh6wgp In reply to <a href='/nevent1qqstm4qd7sj9njrjh6l0fkg0mnu8k0lrejs73nvyutzqyg9vsuqr37cjqkegp'>nevent1q…kegp</a><br/>_________________________<br/><br/>Also, I’m now a bit glad that I’m not grieving for the horrible situation my kids are inheriting. Although I still worry about old infirm me in the future with no kids, maybe AI will take good care of me? 2026-10-11T14:37:43Z https://njump.cloud.vinney.xyz/nevent1qqstm4qd7sj9njrjh6l0fkg0mnu8k0lrejs73nvyutzqyg9vsuqr37czyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9src5p4a In reply to <a href='/nevent1qqsdfcqkgz6m5gmgq232tg3arax4kpht233replxwkn9ftjyzvvzclcc8qllz'>nevent1q…qllz</a><br/>_________________________<br/><br/>Me eager to focus on tenure and worried about what getting pregnant would mean since my new husband still lived a 3hr flight away. Happily did not push back.<br/><br/>But see she was looking at me not my chart. I look young but I was 37 I should have pushed back with my age.<br/><br/>Meditation series convinced me that my regret is grief for a future I thought I would have. But that future never really existed so I’m seeing only one rose colored option not the 100s of less desired possible ones. 2026-10-11T14:35:11Z https://njump.cloud.vinney.xyz/nevent1qqsdfcqkgz6m5gmgq232tg3arax4kpht233replxwkn9ftjyzvvzclczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9s2wl9c3 In reply to <a href='/nevent1qqsgne5adufyx82ljysktx7cynk4e6xlfdt3sz8aphuxed8gkyvz0xq0l3u0y'>nevent1q…3u0y</a><br/>_________________________<br/><br/>Double whammy there! Regret plus fear for the future.<br/><br/>I did a meditation series on regret to help me overcome obsessing about a single split decision that I blame for not having kids.<br/><br/>Shortly after I got married, I asked my doctor about having kids. She brushed it off saying I was young I shouldn’t start worrying about that right now I was young and healthy. 2026-10-11T14:30:47Z https://njump.cloud.vinney.xyz/nevent1qqsd5fmvpwtwsef6autv64c2yy3rv3ldw2980d5xpshews0t9ddf6lgzyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9sy463h2 Mushroom haul from yesterday. Lions mane from indoor bags, shiitake and chestnut from outdoor logs.<br/><br/>#mushtodon<br/> <img src="https://media.mstdn.social/media_attachments/files/117/422/297/445/523/107/original/efd2cb347d880c6a.png"> <br/> 2026-10-11T12:35:52Z https://njump.cloud.vinney.xyz/nevent1qqsqj2pazlvv8ranc37txyx3g88zu78wsvuvym84gxc9ukw7dg0ppsczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9ssn04cw In reply to <a href='/nevent1qqsytfkxz9f979g9mqxsn2m45t69a0yyqglejhjjmtahuavmldfvwcsy8mz9v'>nevent1q…mz9v</a><br/>_________________________<br/><br/>Future dread is the worst. It just gnaws and there’s little you can do except distract yourself.<br/><br/>If it makes you feel any better, a house and a retirement account doesn’t make it go away— particularly thinking about climate change and war or politics wiping out everything.<br/><br/>From your feed,, it seems like you’d be much better off than most if it all goes to hell. 2026-10-11T12:13:16Z https://njump.cloud.vinney.xyz/nevent1qqsxznlqc8dgwu2j95zsk8ns6g7fxcxnp8rjrjyc629lfml7d5zecwczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9sqs4r9w In reply to <a href='/nevent1qqsqqkcgscae7una0vcaewxnguqkveua99xsagx9qtxkzqf7vzhgyychwhhfw'>nevent1q…hhfw</a><br/>_________________________<br/><br/>Do you have untreated sleep apnea? 2026-10-10T14:15:49Z https://njump.cloud.vinney.xyz/nevent1qqs8pl75y949a3f59n08y7nzag8zw98c0l3hpmcue49kc4nejt3w3uqzyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9swcm6v5 In reply to <a href='/nevent1qqs9u8nrrp90nt4prfalwnwsg406na3cjfcvl0a9s44nqz628jj9agcsy5atf'>nevent1q…5atf</a><br/>_________________________<br/><br/>For calculating in lab, I use excel.<br/><br/>I also cross reference to prior calculations, like last time I used 10 microliters why is it 10 ml this time.<br/><br/><span itemprop="mentions" itemscope itemtype="https://schema.org/Person"><a itemprop="url" href="/npub1eeyy9ny4swdppy44gyqx4dffs7mkc7ax7g9lmcelwvtuhcuqmz5qxd339h" class="bg-lavender dark:prose:text-neutral-50 dark:text-neutral-50 dark:bg-garnet px-1"><span>Sam Levine</span> (<span class="italic">npub1eey…339h</span>)</a></span> 2026-10-08T22:48:35Z https://njump.cloud.vinney.xyz/nevent1qqs9u8nrrp90nt4prfalwnwsg406na3cjfcvl0a9s44nqz628jj9agczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9shetpn0 In reply to <a href='/nevent1qqsf8l46eashehguvlmfys72cxjqu65w7g2kd4der8xamqyc9pntg9gc60vsu'>nevent1q…0vsu</a><br/>_________________________<br/><br/>My parents<br/>A) gave me Euclid to catch up on geometry and<br/>b) bought me a typewriter <br/><br/>I have many many self taught work arounds for dsy lexica/calcula which for me are tied.<br/><br/><span itemprop="mentions" itemscope itemtype="https://schema.org/Person"><a itemprop="url" href="/npub1eeyy9ny4swdppy44gyqx4dffs7mkc7ax7g9lmcelwvtuhcuqmz5qxd339h" class="bg-lavender dark:prose:text-neutral-50 dark:text-neutral-50 dark:bg-garnet px-1"><span>Sam Levine</span> (<span class="italic">npub1eey…339h</span>)</a></span> 2026-10-08T22:45:33Z https://njump.cloud.vinney.xyz/nevent1qqsf8l46eashehguvlmfys72cxjqu65w7g2kd4der8xamqyc9pntg9gzyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9ssx44zn In reply to <a href='/nevent1qqsw0fl5ft489mf57hlfnewszpzhfju8a2ct3zlyshpuzk93a0x564qycpkqk'>nevent1q…pkqk</a><br/>_________________________<br/><br/>So in the early 80s when the only term available was dyslexia and I didn’t quite align because I read well for my age but spelling and poor performance in arithmetic were notable enough that I went to special ed/resource room. Eg learning disabled enough for the 80s.<br/> <br/>After two years they told my parents <br/>A) girls didn’t need to be good at math<br/>B) if I could type my bad penmanship wouldn’t be a problem.<br/><br/><span itemprop="mentions" itemscope itemtype="https://schema.org/Person"><a itemprop="url" href="/npub1eeyy9ny4swdppy44gyqx4dffs7mkc7ax7g9lmcelwvtuhcuqmz5qxd339h" class="bg-lavender dark:prose:text-neutral-50 dark:text-neutral-50 dark:bg-garnet px-1"><span>Sam Levine</span> (<span class="italic">npub1eey…339h</span>)</a></span> 2026-10-08T22:41:41Z https://njump.cloud.vinney.xyz/nevent1qqsqgrf9nhze8z84pt90kalj990lcn373jl63fkfcgpdq44pm382rfczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9svn079d In reply to <a href='/nevent1qqswdm5svhhp50ajvf7xlw79xsy2fpwatrl77xhh90flhmsx74upymgezyvnc'>nevent1q…yvnc</a><br/>_________________________<br/><br/>I never cease to be amazed that most bio labs don&#39;t have exact recipes for media and ask people to do calculations. (and also don&#39;t require that people write down what they added so that you can track down problems)<br/><br/>I know this doesn&#39;t help if you misread your own directions.<br/><br/><span itemprop="mentions" itemscope itemtype="https://schema.org/Person"><a itemprop="url" href="/npub1eeyy9ny4swdppy44gyqx4dffs7mkc7ax7g9lmcelwvtuhcuqmz5qxd339h" class="bg-lavender dark:prose:text-neutral-50 dark:text-neutral-50 dark:bg-garnet px-1"><span>Sam Levine</span> (<span class="italic">npub1eey…339h</span>)</a></span> 2026-10-08T20:20:55Z https://njump.cloud.vinney.xyz/nevent1qqswra43anu2r77kzhu5ukh404cly5drthgrv7xh8r48d0lfra79nzszyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9sm6rx4q Back from a two week vacation (ok one week of that was conference but still fun) hubs has been harvesting the garden but not watering or putting bt on the brassicas. Still getting some nice harvest, but probably these are the tail end of squash beans and tomatoes. Note the massive lions mane that hubs totally missed growing on a bag in the garage and two shitakes he missed in the back.<br/> <img src="https://media.mstdn.social/media_attachments/files/117/343/131/854/005/327/original/4f178a498f7a0976.png"> <br/> 2026-09-27T13:03:16Z https://njump.cloud.vinney.xyz/nevent1qqswuqc3vvsy7jxnu3u4p38adah0nrar3afpzlzxzsatv32upwaaa5czyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9s5c92n4 Oof traveling internationally and lost my scrunchy hair tie, my back up hair tie appears to be a dock that I mistook for a hair tie.<br/><br/>And the bonus that my hotel has a bidet is totally counteracted by the fact that it’s a traditional European bidet which seems barbaric compared to a Japanese bidet or even an Indian one. 2026-09-12T20:27:17Z https://njump.cloud.vinney.xyz/nevent1qqsxxc8qqhydj48h5yau5u4dhnpe7eznmunntn2n8lr7qt5rk963jcczyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9sz2w9se Friday’s garden harvest. Expecting a lot more mushrooms today.<br/><br/>We’ve done a terrible job finding bitter gourd before they fully mature. That’s the orange fruit at the back.<br/><br/>Me with one of the shiitake logs.<br/> <img src="https://media.mstdn.social/media_attachments/files/117/184/501/579/180/246/original/ead070936bdb8eb5.png"> <br/> <img src="https://media.mstdn.social/media_attachments/files/117/184/518/585/902/686/original/cf2f95aafd64fa2c.png"> <br/> 2026-08-30T12:45:02Z https://njump.cloud.vinney.xyz/nevent1qqsrftff5uejed49yhhxh7z483fjt6n4832l52vs6tmwj0dxd0y4a7czyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9su0d3c3 what do people with large moveable planters do with them over the winter to prevent weathering? (midwest winters)<br/><br/>My concern is what to do to protect the planter and what to do with the soil. Overwintering plants is not practical. <br/><br/>It’s for eight 25gal wood planters that summer on a deck.<br/><br/>#gardening 2026-08-29T15:49:14Z https://njump.cloud.vinney.xyz/nevent1qqs0svuleemgzfntnudsen38vmxur3y0uylez9prfg0u6pc8fxuxc9szyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9swnj5lg In reply to <a href='/nevent1qqsg24v4k307dnlxzjnrtwk3egtgzdsnl4qw3h2unk3qg59d3g2jjvg7hr8ug'>nevent1q…r8ug</a><br/>_________________________<br/><br/>We got just enough rain to make it unpleasant to sit outside. 2026-08-21T23:37:56Z https://njump.cloud.vinney.xyz/nevent1qqsztv2lxdxpe8puqafzjeajds3h600cjt3967txjjy3j8rs9czdv3czyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9ssupp3n Hi Sciencers.<br/><br/>We&#39;re looking at desmosome components in stem cells and the desmosome proteins don&#39;t always co-localize. Dsp forms bright foci along cell-cell interfaces but only near the apical surface. Dsc and dsg are found along cell-cell interfaces uniformly from apical to basal.<br/><br/>Is this weird?<br/><br/>I know in MDCK cells, they colocalize all along the apical-basolateral interface, I&#39;ve not seen this apical restriction of Dsp reported previously. <br/><br/>But I&#39;m no expert, would love ideas here.<br/> <img src="https://media.mstdn.social/media_attachments/files/117/135/095/336/418/968/original/7b0014908cd53134.png"> <br/> <img src="https://media.mstdn.social/media_attachments/files/117/135/100/923/248/522/original/7f17047d1a68399c.png"> <br/> 2026-08-21T19:17:46Z https://njump.cloud.vinney.xyz/nevent1qqsxsfylucqclt3kkykzprrttgsn29z93vv3mr8sx8kcclccjja33tgzyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9smn0tgz Peak salad season. <br/><br/>Today&#39;s salad-<br/>cherry tomatoes<br/>steamed greens beans<br/>steamed beets<br/>shishitos<br/>mustard greens<br/>kale<br/>chard<br/>beet greens all from the garden<br/><br/>onion, mushrooms, sunflower seeds, dried cranberries, olive oil, salt-not from our garden.<br/><br/>Mustard is the green that keeps giving. Bought in 2020 and still getting enough volunteers in early spring and throughout the summer to supplement any lettuce or other greens we actually plant. Really important in the hot summer when lettuce is rare. 2026-08-12T17:01:17Z https://njump.cloud.vinney.xyz/nevent1qqszurw98jgw4m6lutrfn8p3yd7f3mt9rhse53sk06h8suftd7h3nwqzyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9s8z8xv2 We have to upgrade a computer to the newest window. The computer is attached to an 17 yo plate reader. The software for the plate reader won’t work on the latest windows *and* the upgraded software requires a yearly 1k license fee.<br/><br/>Can a virtual environment for windows work? 2026-08-12T10:56:26Z https://njump.cloud.vinney.xyz/nevent1qqstc53x7dth3lrftlea65zf8yc70rgvwu8ztyr3usnnw723ed3sf3qzyzk6xpnepvjljt0y9a7wccltwklg33gwz0h64u0krs8ukxaaa4f9sdhhd0z In reply to <a href='/nevent1qqsvw7sdqr4t4dfg8vz86kul86at7jn6vpf7atp4fxfzjda7lanxmagaleh52'>nevent1q…eh52</a><br/>_________________________<br/><br/>Tangential. When I was trying to be a positive influence in my brother&#39;s kids lives...the best chance of my genes surviving, and the best chance of anyone caring about me in my dotage...I bought a book about a female scientist for my niece. When wrapping it, I realized it was written by a PhD cohort classmate. 2026-07-31T23:53:07Z